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gpr61 tango plasmid dna  (Addgene inc)


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    Addgene inc gpr61 tango plasmid dna
    Gpr61 Tango Plasmid Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr61+tango+plasmid+dna/GPR61-Tango+(Plasmid+%2366366)/pmc12060159-66-13-17
    Average 93 stars, based on 2 article reviews
    gpr61 tango plasmid dna - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein–coupled receptor GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiT-FZD 6 with a 5-HT 3 A signal peptide plasmid DNA as a backbone ( ). ..

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates. .. Plasmid DNA constructs encoding different HiBiT-GPR61 mutants were generated with a GeneArt Site Directed mutagenesis kit (Thermo Fisher Scientific). rGFP-CAAX plasmid DNA and Gαs-67-RlucII plasmid DNA in the pcDNA3.1(+) backbone were synthesized by GeneScript.

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein-coupled receptor GPR61 occurring in severe obesity.
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiTFZD6 with a 5-HT3A signal peptide plasmid DNA as a backbone (Kozielewicz et al, 2021). ..

    Generated:

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein–coupled receptor GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiT-FZD 6 with a 5-HT 3 A signal peptide plasmid DNA as a backbone ( ). ..

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates. .. Plasmid DNA constructs encoding different HiBiT-GPR61 mutants were generated with a GeneArt Site Directed mutagenesis kit (Thermo Fisher Scientific). rGFP-CAAX plasmid DNA and Gαs-67-RlucII plasmid DNA in the pcDNA3.1(+) backbone were synthesized by GeneScript.

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein-coupled receptor GPR61 occurring in severe obesity.
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiTFZD6 with a 5-HT3A signal peptide plasmid DNA as a backbone (Kozielewicz et al, 2021). ..

    Cloning:

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein–coupled receptor GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiT-FZD 6 with a 5-HT 3 A signal peptide plasmid DNA as a backbone ( ). ..

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates. .. Plasmid DNA constructs encoding different HiBiT-GPR61 mutants were generated with a GeneArt Site Directed mutagenesis kit (Thermo Fisher Scientific). rGFP-CAAX plasmid DNA and Gαs-67-RlucII plasmid DNA in the pcDNA3.1(+) backbone were synthesized by GeneScript.

    Article Title: Identification and molecular characterization of missense mutations in orphan G protein-coupled receptor GPR61 occurring in severe obesity.
    Article Snippet: .. HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using a codon-optimized GPR61 from GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) as an insert and HiBiTFZD6 with a 5-HT3A signal peptide plasmid DNA as a backbone (Kozielewicz et al, 2021). ..



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    gpr61 tango plasmid dna  (Addgene inc)
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    https://www.bioz.com/product/gpr61+tango+plasmid+dna/GPR61-Tango+(Plasmid+%2366366)/pmc12060159-66-13-17
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    RMSD of carbon alpha atoms from the three simulations of the <t>GPR61</t> WT ( A ) and GPR61 R236C ( B ).
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    Image Search Results


    RMSD of carbon alpha atoms from the three simulations of the GPR61 WT ( A ) and GPR61 R236C ( B ).

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: RMSD of carbon alpha atoms from the three simulations of the GPR61 WT ( A ) and GPR61 R236C ( B ).

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques:

    The same procedure was used for GPR61, MC 4 R and GPR141.

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: The same procedure was used for GPR61, MC 4 R and GPR141.

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques:

    A. Mutational landscape of GPR61 in normal population and then the same mutations in severe obesity patients. B. GPR61 mutations mapped onto a 2D model of GPR61.

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: A. Mutational landscape of GPR61 in normal population and then the same mutations in severe obesity patients. B. GPR61 mutations mapped onto a 2D model of GPR61.

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques:

    PolyPhen-2 analysis of the 34 mutations in GPR61 to predict their impact on structure and function of the protein.

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: PolyPhen-2 analysis of the 34 mutations in GPR61 to predict their impact on structure and function of the protein.

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques:

    NanoBiT-based measurement of cell surface expression of 34 mutants of GPR61. The red bars indicate statistically significant decrease and the green bars indicate statistically significant increase in NanoBiT-emmitted cell membrane-associated luminescence indicative of cell surface expression of HiBiT-tagged GPR61 constructs; at least p<0.05 and at least n=3 independent experiments.

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: NanoBiT-based measurement of cell surface expression of 34 mutants of GPR61. The red bars indicate statistically significant decrease and the green bars indicate statistically significant increase in NanoBiT-emmitted cell membrane-associated luminescence indicative of cell surface expression of HiBiT-tagged GPR61 constructs; at least p<0.05 and at least n=3 independent experiments.

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques: Expressing, Membrane, Construct

    A. EPAC-derived FRET-based biosensor reveals that three mutations (the red bars) lead to the statistically significant reduction in cAMP production elicited by overexpression of a GPR61 in the absence of an agonist (increase in the FRET ratio reflects decrease in cAMP levels). B. The presence of overexpressed GPR61 R236C leads to a statistically-significant reduction in the bystander BRET ratio between Gαs-67-RlucII and rGFP-CAAX indicative of reduced G s translocation away from the cell membrane; at least p<0.05 and at least n=3 independent experiments.

    Journal: medRxiv

    Article Title: Identification and molecular characterization of missense mutations in orphan GPCR GPR61 occurring in severe obesity

    doi: 10.1101/2024.10.21.24315834

    Figure Lengend Snippet: A. EPAC-derived FRET-based biosensor reveals that three mutations (the red bars) lead to the statistically significant reduction in cAMP production elicited by overexpression of a GPR61 in the absence of an agonist (increase in the FRET ratio reflects decrease in cAMP levels). B. The presence of overexpressed GPR61 R236C leads to a statistically-significant reduction in the bystander BRET ratio between Gαs-67-RlucII and rGFP-CAAX indicative of reduced G s translocation away from the cell membrane; at least p<0.05 and at least n=3 independent experiments.

    Article Snippet: HiBiT-GPR61 plasmid DNA was generated with Gibson cloning using GPR61-Tango plasmid DNA (#66366 Addgene, deposited by Bryan Roth) and HiBiT-FZD 6 plasmid DNA as templates.

    Techniques: Derivative Assay, Over Expression, Translocation Assay, Membrane